competent e coli (TaKaRa)
97
Structured Review
TaKaRa
competent e coli
Competent E Coli, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stellar+competent+e+coli+cells/Stellar+Competent+Cells/bio_rxiv__64898__2026__05__10__724078-211-8-11
Average 97 stars, based on 1475 article reviews
Competent E Coli, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stellar+competent+e+coli+cells/Stellar+Competent+Cells/bio_rxiv__64898__2026__05__10__724078-211-8-11
Average 97 stars, based on 1475 article reviews
competent e coli - by Bioz Stars,
2026-09
97/100 stars
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Polymerase Chain Reaction:Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases. Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Article Title: Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination Article Snippet: Rad54 deep mutagenesis screen RAD54 and 500-bp upstream sequence was cloned into pRS415 (centromeric origin, LEU2 marker) by generating three fragments by PCR amplification (PrimeSTAR Max DNA Polymerase, Takara Bio, Cat. No. R045B): two from RAD54 S. cerevisiae W303 genomic DNA covering +500-RAD54 with an overlap region producing a silent mutation at K152 (A456G) to disrupt a 10-adenine tract with oligonucleotide primers MTP85/MTP135 and MTP86/MTP134; and a third PCR fragment from purified pRS415 with oligonucleotide primers VBR15/VBR16 (all oligonucleotide sequences are presented in Dataset 1). .. PCR products were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases. Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using In-Fusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Transformation Assay:Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases. Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Article Title: Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination Article Snippet: Rad54 deep mutagenesis screen RAD54 and 500-bp upstream sequence was cloned into pRS415 (centromeric origin, LEU2 marker) by generating three fragments by PCR amplification (PrimeSTAR Max DNA Polymerase, Takara Bio, Cat. No. R045B): two from RAD54 S. cerevisiae W303 genomic DNA covering +500-RAD54 with an overlap region producing a silent mutation at K152 (A456G) to disrupt a 10-adenine tract with oligonucleotide primers MTP85/MTP135 and MTP86/MTP134; and a third PCR fragment from purified pRS415 with oligonucleotide primers VBR15/VBR16 (all oligonucleotide sequences are presented in Dataset 1). .. PCR products were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases. Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using In-Fusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Article Title: The human DEAD-box protein DDX3X regulates host and viral mRNA translation during Sendai Virus infection Article Snippet: .. Inserts were cloned into the vector using the In-Fusion® Snap Assembly kit (Takara Bio, 638943) and transformed into Article Title: Suppression of astrocyte BMP signaling improves molecular signatures and functional deficits in a fragile X syndrome mouse model. Article Snippet: .. Cloning:Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains Article Snippet: .. Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains. Article Snippet: .. Methylation:Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains Article Snippet: .. Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains. Article Snippet: .. Clone Assay:Article Title: The human DEAD-box protein DDX3X regulates host and viral mRNA translation during Sendai Virus infection Article Snippet: .. Inserts were cloned into the vector using the In-Fusion® Snap Assembly kit (Takara Bio, 638943) and transformed into |