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competent e coli  (TaKaRa)


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    Structured Review

    TaKaRa competent e coli
    Competent E Coli, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stellar+competent+e+coli+cells/Stellar+Competent+Cells/bio_rxiv__64898__2026__05__10__724078-211-8-11
    Average 97 stars, based on 1475 article reviews
    competent e coli - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases.
    Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Stellar competent E. coli cells (Takara Bio, Cat. No. 636763). ..

    Article Title: Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination
    Article Snippet: Rad54 deep mutagenesis screen RAD54 and 500-bp upstream sequence was cloned into pRS415 (centromeric origin, LEU2 marker) by generating three fragments by PCR amplification (PrimeSTAR Max DNA Polymerase, Takara Bio, Cat. No. R045B): two from RAD54 S. cerevisiae W303 genomic DNA covering +500-RAD54 with an overlap region producing a silent mutation at K152 (A456G) to disrupt a 10-adenine tract with oligonucleotide primers MTP85/MTP135 and MTP86/MTP134; and a third PCR fragment from purified pRS415 with oligonucleotide primers VBR15/VBR16 (all oligonucleotide sequences are presented in Dataset 1). .. PCR products were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Stellar competent E. coli cells (Takara Bio, Cat. No. 636763). ..

    Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases.
    Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using In-Fusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Stellar competent E. coli cells (Takara Bio, Cat. No. 636763). ..

    Transformation Assay:

    Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases.
    Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Stellar competent E. coli cells (Takara Bio, Cat. No. 636763). ..

    Article Title: Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination
    Article Snippet: Rad54 deep mutagenesis screen RAD54 and 500-bp upstream sequence was cloned into pRS415 (centromeric origin, LEU2 marker) by generating three fragments by PCR amplification (PrimeSTAR Max DNA Polymerase, Takara Bio, Cat. No. R045B): two from RAD54 S. cerevisiae W303 genomic DNA covering +500-RAD54 with an overlap region producing a silent mutation at K152 (A456G) to disrupt a 10-adenine tract with oligonucleotide primers MTP85/MTP135 and MTP86/MTP134; and a third PCR fragment from purified pRS415 with oligonucleotide primers VBR15/VBR16 (all oligonucleotide sequences are presented in Dataset 1). .. PCR products were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using InFusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Stellar competent E. coli cells (Takara Bio, Cat. No. 636763). ..

    Article Title: Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases.
    Article Snippet: .. Following the PCR reactions, the samples were digested with DpnI (20 units; NEB, Cat. No. R0176), assembled using In-Fusion Snap Assembly (Takara Bio, Cat. No. 638947) and transformed into Stellar competent E. coli cells (Takara Bio, Cat. No. 636763). ..

    Article Title: The human DEAD-box protein DDX3X regulates host and viral mRNA translation during Sendai Virus infection
    Article Snippet: .. Inserts were cloned into the vector using the In-Fusion® Snap Assembly kit (Takara Bio, 638943) and transformed into Stellar competent E. coli cells (Takara Bio, 636766) as per manufacturer’s instructions. .. All constructs were sequence-verified using Sanger sequencing (MWG Eurofins), and the sequences of inserts are detailed in Table S3.

    Article Title: Suppression of astrocyte BMP signaling improves molecular signatures and functional deficits in a fragile X syndrome mouse model.
    Article Snippet: .. Stellar competent E. Coli cells (Takara 636763) were then transformed with the plasmids via heatshock according to the manufacturer’s instructions. .. Bacteria were screened using carbenicillin resistance (100 μg/mL; Teknova C2130).

    Cloning:

    Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains
    Article Snippet: .. Stellar competent E. coli cells (Takara Bio) are highly competent cloning cells that lack methylation gene ( dam- / dcm- ) and are deficient in methylated DNA restriction ( mrr-hsdRMS-mcrBC and mcrA ). .. Stop Unwanted Rearrangement Events (SURE) competent cells (Agilent Inc.) are restriction minus ( McrA-, McrCB-, McrF-, Mrr-, HsdR- ), endonuclease endA deficient and have a recB and recJ deficiency.

    Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains.
    Article Snippet: .. Stellar competent E. coli cells (Takara Bio) are highly competent cloning cells that lack methylation gene (dam-/dcm-) and are deficient in methylated DNA restriction (mrr- ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS hsdRMS-mcrBC and mcrA). .. Stop Unwanted Rearrangement Events (SURE) competent cells (Agilent Inc.) are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-), endonuclease endA deficient and have a recB and recJ deficiency.

    Methylation:

    Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains
    Article Snippet: .. Stellar competent E. coli cells (Takara Bio) are highly competent cloning cells that lack methylation gene ( dam- / dcm- ) and are deficient in methylated DNA restriction ( mrr-hsdRMS-mcrBC and mcrA ). .. Stop Unwanted Rearrangement Events (SURE) competent cells (Agilent Inc.) are restriction minus ( McrA-, McrCB-, McrF-, Mrr-, HsdR- ), endonuclease endA deficient and have a recB and recJ deficiency.

    Article Title: SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains.
    Article Snippet: .. Stellar competent E. coli cells (Takara Bio) are highly competent cloning cells that lack methylation gene (dam-/dcm-) and are deficient in methylated DNA restriction (mrr- ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS hsdRMS-mcrBC and mcrA). .. Stop Unwanted Rearrangement Events (SURE) competent cells (Agilent Inc.) are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-), endonuclease endA deficient and have a recB and recJ deficiency.

    Clone Assay:

    Article Title: The human DEAD-box protein DDX3X regulates host and viral mRNA translation during Sendai Virus infection
    Article Snippet: .. Inserts were cloned into the vector using the In-Fusion® Snap Assembly kit (Takara Bio, 638943) and transformed into Stellar competent E. coli cells (Takara Bio, 636766) as per manufacturer’s instructions. .. All constructs were sequence-verified using Sanger sequencing (MWG Eurofins), and the sequences of inserts are detailed in Table S3.



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